RNA

Part:BBa_K4061045:Design

Designed by: Raissa Gavrila Dani   Group: iGEM21_HKUST   (2021-09-30)


mRFP1 antisense to downregulate mRFP1 (BBa_E1010)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 49
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We would have to design the sequence such that it has a low complexity, thus making it eligible to be synthesized by IDT


Source

The antisense fragment is customized to downregulate mRFP1 (BBa_E1010). Thus, the sequence was designed in accordance to the coding region of mRFP1

References